To simulate alterations in immune homeostasis with frequent IFN-I production and to test whether the IFN-I signature and protective adaptive responses we observe in CB4-infected MDA5+/- mice can be maintained despite additional bursts of IFN-I, we stimulated CB4-infected MDA5+/- and MDA5+/+ mice 3 and 5 days post-CB4 infection with the dsRNA mimetic poly I:C. The gene discussed is IFIH1; the disease is infection.