Similar to previous results, we determined that honey bees fed sucrose containing clothianidin (1 ppb) had reduced expression of key immune genes (i.e., ago2, dcr-like, abaecin, hymenoptaecin, and vitellogenin), which resulted in more severe virus infections, using virus abundance as a proxy for infection severity for a panel of viruses. This evidence concerns the gene AGO2 and viral infectious disease.