In the present study, ELISA assay showed that the active flavonoids could significantly decrease the levels of proinflammatory cytokines, TNF-α and IL-1β, in the joint tissue fluid of MSU crystal-induced rats, indicating that luteolin, luteoloside, and apigenin participate in the regulation of inflammatory factors, which mediates the pathophysiology of gouty arthritis in MSU crystal-induced rats. The gene discussed is TNF; the disease is gout.