Following TAC surgery, the ratio of p-ERK1/2 to ERK1/2 in the myocardial tissue of the AG/TAC group increased significantly, while GPR30/G1 can significantly reduce the ratio of p-ERK1/2 to ERK1/2 in the myocardial tissue compared to the AG/TAC group (Figure 5C). The gene discussed is MAPK3; the disease is persistent truncus arteriosus.