TGFB1 and persistent truncus arteriosus: Western blot results revealed that continuous intraperitoneal injection of GPR30 agonist G1 for 8 weeks combined with GPR30 overexpression affected the expressions of GPR30, p-ERK, ERK, MMP-9, and TGF-β1 proteins in the myocardium of aged TAC mice (Figure 5A).