Taken together, these results suggest that NSC/JQ1 combination suppresses breast cancer cells growth and tumorigenesis by targeting multiple signaling pathways including induction of autophagy via LC3B, disruption of c-MYC-G9a-FTH1 axis, modulation of histone modification and chromatin remodeling by targeting HDAC1/Ac-H3K9 axis in different molecular subtypes of breast cancer. This evidence concerns the gene HDAC1 and breast carcinoma.