To verify the role of FBW7/EZH2/ZBTB16 axis in T1D, we transfected the sh-ZBTB16 vector to the Min6 cells, and the silencing efficiency was confirmed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) with sh-ZBTB16-1 showed the best efficiency selected in the following experiments (Fig. 5A). The gene discussed is EZH2; the disease is type 1 diabetes mellitus.