Due to the change of lysosome membrane protein and the integrity with Dcf1, accelerated the release of inclusion in the lysosomal lumen to the cytosol, here, we observed that the level of lysosomal aspartyl protease, Cathepsin D, acid phosphatase, Cathepsin B in cytoplasm was increased after isolated the lysosome from glioblastoma cells (Figure 5G; Figure S5D,E, indicated by the arrow). Here, TMEM59 is linked to glioblastoma.