Importantly, TCF7L1, IL-8, CXCR2, and NE markers decreased, and androgen-responsive markers increased in DHT-treated cells (Fig. 3E, F), which supports TCF7L1/IL-8/CXCR2 upregulating NED function in PCa cells as observed post-ADT. This evidence concerns the gene CXCL8 and posterior cortical atrophy.