PROM1 and neoplasm: To trace the gradual change in metabolism in bulk tumor cells resulting from CSCs, we generated an HT29-CD133-RFP cell line by inserting an RFP sequence with the 2A self-cleaving peptide at the stop codon of the gene encoding CD133 in CSCs using the CRISPR/Cas9 knock-in system (Supplementary Fig. S1).