Nevertheless, we found that trastuzumab treatment could upregulate IFN-γ production in the supernatant of the coculture of PBMCs with either tumor cell line (Fig. 5E), and IFN-γ treatment could elevate the expression of PD-L1 in both HCC1954 and NCI-N87 cells (Fig. 5F). This evidence concerns the gene CD274 and neoplasm.