Fig 5 indicated that the expression levels of the 13 hub genes were significantly higher in breast cancer tissues than in normal breast tissues (P < 0.05). Subsequently, the KM plotter was adopted to the survival analysis for mentioned 12 hub genes (CDK1 data was not found), resulting that only the CCNB1 was associated with statistical poor survival (P<0.05) in TNBC (Fig 6). Eventually, THAP database was employed to explore the relative expression of CCNB1 in BC. Immunohistochemistry (IHC) in THPA database verified that CCNB1 was upregulated in breast cancer tumor tissues (Fig 7). This evidence concerns the gene CCNB1 and breast carcinoma.