Under these conditions, NAD+ levels are approximately halved in cells expressing exogenous WT SARM1 (due to overexpression of a low constitutive NADase activity) but we observed a substantially greater lowering of NAD+ for six of the ALS-specific variants - L223P, Δ229–235 (alternatively named Δ226–232 - see Table 1 legend), Δ249–252, V331E, E340K, and T385A SARM1 - suggestive of strong NADase GoF (Figure 2A). The gene discussed is SARM1; the disease is amyotrophic lateral sclerosis.