The increase in activity of WT SARM1 in the presence of 10 μM NMN was roughly half that seen with 50 μM NMN (in parallel assays) and all the ALS patient variants previously found to be responsive to 50 μM NMN showed a similar or even lower relative level of activation with 10 μM NMN (Figure 3D and Figure 3—figure supplement 2B,D), apart from V112I SARM1 which again is not inducible (Figure 3D). This evidence concerns the gene SARM1 and amyotrophic lateral sclerosis.