Since BRL52537, a kappa-opioid receptor agonist, could regulate p-STAT3 but not STAT3 expression in cerebral IRI and is involved in kidney injury, we used BRL52537 to assess whether p-STAT3 is necessary for the protective effects of BA (Fang et al., 2013; Golosova et al., 2020). The gene discussed is OPRK1; the disease is kidney injury.