The detected variants were found in nine genes (COL4A5, DIAPH1, MYO7A, MYO15A, OTOGL, TECTA, TMC1, TMPRSS3, TSPEAR) harbouring four pathogenic variants, three likely pathogenic variants and nine VUS variants associated with hearing impairment. Here, COL4A5 is linked to Hearing impairment.