To assess G3BP1-SPOP signaling in SPOP-mutant PCa, we generated Pten−/−-SPOPF133V mPECs (Supplementary Fig. 7C) with doxycycline (DOX)-inducible G3BP1 as a model of G3BP1high SPOPF133V PCa. Here, SPOP is linked to posterior cortical atrophy.