Using a 56-marker CODEX panel (Fig. 1e, Supplementary Fig. 1e, Supplementary Table 2), unsupervised machine learning, and manual curation based on marker expression, tissue localization, and morphology, 21 unique cell types were identified and validated (Fig. 1f, Supplementary Fig. 2a, b), including reactive CD4+ T cells and malignant CD4+ T cells (i.e., tumor cells). The gene discussed is CD4; the disease is neoplasm.