Interestingly, only three genes (BRD3, TWF2, and ZNF365) were differentially expressed in nonresponder tumor cells post-treatment compared to pretreatment (Fig. 5j), which suggests that nonresponder tumor cells are resistant to PD-1 blockade, in line with the increased tumor therapy resistance score observed in nonresponders post- vs pretreatment (Fig. 4h). The gene discussed is PDCD1; the disease is neoplasm.