LIPA and metabolic dysfunction-associated steatotic liver disease: Based on this background, in the present study, we have explored LAL protein content as well as activity both in in vitro (fat-loaded hepatocyte-derived human carcinoma Huh7 cells) and in vivo (mice fed with high-fat diet, HFD) experimental models of NAFLD in order to clarify the mechanisms underlying LAL activity impairment in NAFLD.