NF1 and hepatocellular carcinoma: To further consolidate our CRISPR/Cas9 knockout library screening result, we constructed knockout and RNAi cell lines of NF1 and DUSP9 in two HCC cells, Huh7 and PLC/PRF/5 (Fig. 3A, B), while the Kras (G12V) overexpression cell line served as a positive control (Fig. 3C).