To determine the effects of GOLM1 on macrophages and PD-L1 expression in vivo in HCC, we established subcutaneous implantation models in C57BL/6 mice using Hepa1-6 transfected with shGOLM1 (mouse, shGOLM1#2) or non-target shRNA control (shNT) (Supplementary Fig. S2a). The gene discussed is CD274; the disease is hepatocellular carcinoma.