CD8+ T cells from Hepa1-6 shGOLM1 tumor exhibited low PD-1 and TIM-3 expression relative to Hepa1-6 shNT tumor, with increased effector cytokines, IFN-γ and GZMB (Fig. 2c and Supplementary Fig. S2b). This evidence concerns the gene PDCD1 and neoplasm.