CD274 and hepatocellular carcinoma: To further evaluate the functional roles of GOLM1 on the PD-L1 level of HCC cells, we generated three GOLM1-specific short hairpin RNAs (shRNAs) mediated by lentivirus to knockdown (KD) GOLM1 in HCC cells (MHCC-97H and HCC-LM3 cells), shGOLM1#3, which induced the most significant knockdown effect, was adopted for further studies.