Flow cytometry analysis of macrophages and TAMs infiltration demonstrated that GOLM1-KD significantly decreased the population of macrophages and TAMs but did not alter the population of CD8+ T cells and their proliferation (Ki67) significantly, which was consistent with that in human HCC tissues (Fig. 2a and Supplementary Fig. S2b). The gene discussed is MKI67; the disease is hepatocellular carcinoma.