In this study we show, using a conscious guinea pig model of cough, that acute exposure to PGE2, via the central route, results in sensitization of the cough reflex which is mediated via EP3 receptor-dependent activation of NaV 1.8 channels but independently of TRPV1, TRPA1 or TTX-sensitive channel activation. The gene discussed is TRPA1; the disease is Cough.