At 24 and 48 h, more ICG fluorescence signal was observed in the tumors of ICG@PM@NP or ICG@NP treated mice when compared with other normal tissues like heart and lung, which may be induced by enhanced permeability and retention effect and the active tumor-targeting ability of albumin (Fig. 5A).[24, 40, 45] Following this, UV–VIS and HPLC–MS were used to more accurately evaluate the accumulation of ICG and PM in tumors, respectively (Fig. 5B–E). Here, ALB is linked to neoplasm.