Anti-PD1 treatment alone has no obviously therapeutic effects against tumor growth, and the main reason may be the rare CD8+ T infiltration in tumor, especially the lack of antigen-specific CD8+ T. On the control, gCpG based tumor vaccine treatment dramatically enhanced tumor-infiltrating CD8+ T, especially antigen-specific CD8+ T, and the combination of anti-PD1 treatment and gCpG based tumor vaccine thus dramatically enhanced the therapeutic efficacy of tumor growth. Here, PDCD1 is linked to neoplasm.