We confirmed by semi-quantitative RT-PCR (S5C Fig) that the transcription of TgNFS2 and TgISU1 qenes was effectively repressed in the cKD cell lines upon addition of ATc, whereas the corresponding complemented cell lines exhibited a high transcription level regardless of ATc addition (due to the expression from the strong tubulin promoter). The gene discussed is ATM; the disease is chronic kidney disease.