We then generated four groups of mice (Trp53fl;Rsk2+/0; Trp53Cre;Rsk2+/0; Trp53fl;Rsk2-/0; Trp53Cre;Rsk2-/0) with the aim to analyze osteosarcoma progression. The gene discussed is RPS6KA3; the disease is osteosarcoma.