PPI links connecting to key metabolic enzymes, such as RRM2, NDUFA11, ENO2, EZH2, AKR1C2, and TYMS, are identified from signaling proteins (e.g., PPARD, BAD, GNB5, CHECK1, PAK2, PLK1, BRCA1, MAML3, and SPP1), TFs (e.g., KAT2B, ING1, MED1, ZEB1, AR, NCOA2, EGR1, TWIST1, E2F1, ID4, RBL1, ESR1, and HSF2), and miR (e.g., mir-147a, mir-593-5p, mir-138-5p, mir-16-5p, and mir-15b-5p) in cervical cancer scenario. The gene discussed is TWIST1; the disease is cervical cancer.