The strongest T1D susceptibility genes HLA-DQB1 and GA62 (encodes GAD65), the T1D-related inflammatory cytokine TNF and the TLR receptor signaling pathway-related protein TRAF6 are representative MVPs found in this analysis; additionally, some of these MVPs were found to be altered prior to overt T1D onset and maintained temporal stability over many years, which may provide a potential possibility of early clinical diagnosis of T1D (Rakyan et al., 2011; Cerna, 2019). Here, TNF is linked to type 1 diabetes mellitus.