Among them, normal follicular epithelial cells (NFECs) from PCa were identified by TG, EpCAM, TPO, etc. marker genes, malignant follicular epithelial cells (MFEC)s from Ca and LN by TG, EpCAM, CK19/KRT19, MET, etc. marker genes, and interstitial cell subsets from the three samples by their respective marker genes, such as CD4+ T (Th) cells (CD4+, CD25-)/CD4+ T (Treg) cells (CD4+, CD25+), CD8+ T cells (CD8), B cells (CD19, CD20), vascular endothelial cells (VWF, ACKR1), dendritic cells (LAMP3), fibroblasts (ACTA2, FN1, THY1, TAGLN), and CAFs (FAP, POSTN, CXCL12) (Supplementary Figures S1A, B). The gene discussed is ACTA2; the disease is posterior cortical atrophy.