CD4 and neoplasm: Among CD3+ T cells, 44.3% were CD8+ and 43.5% were CD4+ T cells without co-culturing with target tumor cells, while 63% were CD8+ and 27.9% were CD4+ T cells after co-culturing with SW480 (Figure 4I), suggesting that CD8+ T cells may play a more important role in specific cytotoxicity.