Genetic epidemiological and experimental data all suggest a critical role of gliosis and neuroinflammation in AD.56,57 To study gliosis in AppNL-G-F rats, we first measured the protein levels of Iba1 (a microglia marker) and GFAP (an astrocyte marker) in AppNL-G-F rats. The gene discussed is GFAP; the disease is Alzheimer disease.