Of note, co-operation of phosphatidylinositol 3-kinase (PI3K)/AKT, Notch, and NO signalling has been reported during tumourigenesis in a Drosophila eye cancer model21 and NO can have both pro- and anti-tumourigenic effects, depending on NO levels and cancer types22,23. The gene discussed is AKT1; the disease is ocular cancer.