Next, we investigated if chimeric OSRs can be used to engineer primary T cells and confer cytotoxic ability because the use of engineered T cells expressing cancer-targeting TCRs or chimeric antigen receptors for immunotherapy has gained significant attention.31 PBMC-derived CD8 + T cells from a healthy donor were engineered to express a chimeric TCR generated with OSR6, the dominant TCR identified in Pt10 opT cells (figure 4B). This evidence concerns the gene CD8A and cancer.