qRT-PCR analysis revealed that the expression levels of inflammatory proinflammatory cytokines (TNF-α, IL-6, and IL-1β) and the inflammatory chemokine CCL-2, which were enhanced in the hepatic tissues of mice with NAFLD, were significantly inhibited by circRNA_0001805 overexpression (Fig. 6F). This evidence concerns the gene TNF and metabolic dysfunction-associated steatotic liver disease.