LGALS3 and malignant colon neoplasm: In addition, LSCM results showed a large amount of red fluorescence localized on the cell membrane of HCT116 cell, HCT116/Fu cell, HCT116/L cell, HT-29 cell and SW620 cell (Fig. 1D), indicating that Gal-3 highly expressed on the cell membrane of colon cancer cell.