Western blot results indicated that in comparison with normal colon epithelial cells (NCM460 cells), Gal-3 protein expression was significantly increased in colon cancer cell line such as HCT116 cell, HCT116/Fu cell, HCT116/L cell, HT-29 cell and SW620 cell (Fig. 1C). This evidence concerns the gene LGALS3 and colonic neoplasm.