Furthermore, scRNA-seq analysis indicated that the Keratin19 (Krt19)+ radioresistant stem cell marker, the reserve stem cell markers Hopx and Lrig1 and the Ascl2 and Msi1 transcripts that are required for the activation of the reserve stem cells were strongly increased in the Lef1-deleted adenoma cells (Fig. 5K) (52–56). This evidence concerns the gene ASCL2 and adenoma.