We further validated these findings by stable expression of two different short hairpin RNAs (shRNAs) designed to target RBM39 in four different neuroblastoma models, all of which are MYC-driven as a result of either MYCN amplification (BE2C, KELLY, and SIMA) or C-MYC overexpression (SK-N-AS) (Fig. 1E). This evidence concerns the gene MYCN and neuroblastoma.