Thus, the above data support that SARS-CoV-2 virus from 16HBE (ACE2) cells binds to DC- and L-SIGN receptors via its high-mannose glycan present at the N1098 site in the postfusion S trimer to enhance viral infection in cis and trans. On the other hand, the binding of virions from Vero, 293T, and A549 (ACE2) cells to DC- and L-SIGN receptors is mainly dependent on high-mannose glycans present in the S1 subunit of the prefusion trimer (Figure 4D). Here, ACE2 is linked to viral infectious disease.