As expected, MTHFR was again depleted (Fig. 3A, right plot) but we did not detect any dramatic changes in either histone methylation or CRC cell growth and viability (Fig. 3B, compare lanes 3 and 4 with lanes 1 and 2, Fig. 3C, and Fig. 3D top images). The gene discussed is MTHFR; the disease is colorectal carcinoma.