Given that we identified TYMS to be significantly downregulated upon NAA40-knockdown (Fig. 1A, C) and the fact that Cancer Dependency Map (DepMap) data analysis shows significant positive correlation (r = 0.43, p < 0.001) between NAA40 and TYMS expression in 70 different CRC cell lines (Supplementary Fig. S6A), we speculated that NAA40 could be a novel regulator of TYMS affecting the response of CRC cells to 5-FU. Here, NAA40 is linked to cancer.