UBE2S and hepatocellular carcinoma: CCK-8 assays were used to examine the cytotoxic effects of 5-FU and oxaliplatin on these cell lines, showing that PLC/PRF/5, Huh7, and MHCC-97L cells were more resistant to oxaliplatin and 5-FU compared with Hep3B and HepG2 cells (Fig. 2B–E), indicating that the level of UBE2S is associated with the resistance of HCC cells to chemotherapy agents.