Additionally, in the GSEA results upon the TCGA RNA-seq data, we found that genes upregulated in cancer cells upon knockdown of PTEN were enriched in HCC tissues with high expression levels of UBE2S or FOXM1, while genes downregulated in PTEN-knockdown cancer cells were enriched in HCC tissues with low expression levels of UBE2S or FOXM1 (Fig. 5A–D). This evidence concerns the gene PTEN and hepatocellular carcinoma.