As the result in Fig. 5I–M showed that PTEN level was negative correlated with the HCC cell chemoresistance, we subjected the UBE2S overexpressed together with indicated PTEN wild-type and corresponding mutant overexpressed HCC cells to CCK-8 assays after 5-FU and oxaliplatin treatment. The gene discussed is PTEN; the disease is hepatocellular carcinoma.