MAP2 and Roberts-SC phocomelia syndrome: Next, in order to assess immunohistochemical differences of RBS lesions compared to control retinas in an impartial way, we quantified immunostaining signals digitally using the Fiji/ImageJ software [28] The sheet-like growing cells of RBS lesions and the GCL of the control retina, which corresponded in morphology and MAP2C positivity, were designated as the cellular compartments eligible for quantification in both tissues (Fig. 4a–o).