We then used ChIP-qPCR to determine the distribution of TOP2β and ERα over the translocation sites in ten Xp11.2 tRCC patients, using HK-2 cells with or without etoposide or E2 treatment respectively. The gene discussed is ESR1; the disease is renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.