CD4+ CD25+ T cells increased in the untreated tumours of mice treated with radiation alone; however, this increase in intratumoural Tregs was abrogated in the combination-treated mice, leading to an overall significant increase in the intratumoural CD8+ T cell-to-Treg ratio in mice treated with the combination therapy (figures 2d,e). This evidence concerns the gene CD8A and neoplasm.