Treatment of crypts from patients with obesity, independent of TAS2R43 genotype, with 1 mM DB resulted in 120 DEGs compared with control-treated (DMEM-treated) crypts, as identified by an adjusted P value (q) of less than 0.05 (Figure 7A and Supplemental Figure 6). This evidence concerns the gene TAS2R43 and obesity due to melanocortin 4 receptor deficiency.