Furthermore, only in the ER+ group a proportional increase in metastatic disease was observed with the increase in HSF1 expression (Figure 6H) and metastatic tumors were overrepresented in ER+/HSF1high (62% versus 44.3% in ER+/HSF1low) (p-value=0.059, Pearson’s chi-squared test, verified by chi-squared posthoc test) (Figure 6G, bottom). This evidence concerns the gene ESR1 and metastatic neoplasm.