We found that PI3K‐110α, p‐mTOR and p‐AKT, N‐cadherin, and Vimentin levels were decreased after PB28 was added, indicating that PB28 binding to its receptor TMEM97 could inhibit the subunit of PI3K, phosphorylation of AKT and mTOR (AKT and mTOR were not influenced) to suppress renal cancer cell growth and metastasis. This evidence concerns the gene VIM and renal carcinoma.