To explore the mechanisms underlying XB130 expression, the possible downstream targets of XB130 including PI3K downstream (Akt), Ser473 phosphorylated Akt (pAkt) and epithelial-mesenchymal transition (EMT) markers (E-cadherin and vimentin) were measured in the XB130 knockdown-CCA cell lines using western blot analysis. Here, AFAP1L2 is linked to cholangiocarcinoma.