Based on the transcriptional changes proposed in the liver steatosis AOP, and based on the NR activation pattern of PHX, PHP, and CTD, we investigated the expression of ACOX1, FASN, MLXIPL, SCD and SREBF1. Additionally, we included other genes related to xenobiotic metabolism, hepatotoxicity and NR activation. The gene discussed is SREBF1; the disease is fatty liver disease.