YBX1 and Hyperglycemia: Consistent with hyperglycemia-induced reduced expression of YB-1 in H9c2 cells and in diabetic mice, Co-IP studies showed an impaired interaction between YB-1 and OTUB1 in H9c2 cells incubated with HG, whereas aPC treatment significantly preserved YB-1/OTUB1 interaction (Figure 4G; Supplementary Figure S6D).