The depletion of Runx2 led to a statistically significant ~50% decrease in calcification in both the valve sinus wall and leaflet hinge region of the LDLr−/−ApoB100Runx2ΔSMA when compared with the LDLr−/−ApoB100Runx2f/f 26 weeks on the T2DM diet mice (Figures 7B,C, respectively). This evidence concerns the gene RUNX2 and type 2 diabetes mellitus.