The LDLr−/−ApoB100Runx2ΔSMA and control LDLr−/−ApoB100Runx2f/f mice on the T2DM diet were treated with tamoxifen from week 6 through 8 to induce Cre activation and Runx2 depletion specifically in the aVICs and sinus wall cells expressing SMA. Here, SMN1 is linked to type 2 diabetes mellitus.