To determine whether the transient depletion of Runx2 in the SMA+ aVICs and sinus wall cells improved the aortic valve hemodynamic function, we performed an echocardiographic analysis of the LDLr−/−ApoB100Runx2ΔSMA (n = 20) and LDLr−/−ApoB100Runx2f/f (n = 25) mice after 26 weeks on the T2DM diet (n = 20) and LDLr−/−ApoB100Runx2f/f on NC diet (n =18). The gene discussed is RUNX2; the disease is type 2 diabetes mellitus.