As shown in Figures 6A,B, the mRNA expression and the protein level of TP53 were downregulated by sh-TP53 in ESCC cells, which in turn significantly reduced the growth rate of Kyse150 and TE1 cells, as assessed by cell proliferation assay (Figures 6C,D). The gene discussed is TP53; the disease is esophageal squamous cell carcinoma.