CD40LG and neoplasm: As expected, combinational therapy (chemo/immuno) induced various immune infiltration methods into the tumor regions manifested by an elevated expression of many immune markers, including CD4, CD68, GZMB, CD40LG (CD40 ligand), CD86, CD276, and LAG3 (lymphocyte-activation gene 3) at the mRNA level in multiple tumor ROIs (Figure 2D).